四氯化碳、前列腺素和乙二胺四乙酸对大鼠肝匀浆不同孵育时间谷胱甘肽水平的“体外”影响

["In vitro" effect of CCl4, PG and EDTA on GSH levels at different time of incubation of rat liver homogenates].

作者信息

Rossi M A, Fenili S, Dianzani M U

出版信息

Boll Soc Ital Biol Sper. 1983 Jan 31;59(2):245-51.

Abstract

During CCl4-induced lipid peroxidation GSH content in total homogenate from rat liver falls very rapidly in the first 30 min. of incubation "in vitro". CCl4 does not enhance the decrease in total glutathione (TG) during the incubation time, so GSH loss is mainly due to its oxidation to GSSG. On the contrary PG and EDTA, two substances decreasing lipid peroxidation rate, are able to decrease GSH oxidation, without affecting TG content. At 25 degrees C EDTA and PG completely prevent GSH decrease at pH 7.4, while at pH 6 PG affords only a partial prevention. At 37 degrees C both compounds are able to limit GSH decrease at a large extent. Lipid peroxidation seems to have a great importance in the kinetics of GSH decrease and GSSG formation, at least "in vitro". It is noteworthy that PG which inhibits lipid peroxidation stimulated by CCl4 is also able to limit the high GSH loss observed in the homogenates incubated in the presence of halogeno-alkane.

摘要

在四氯化碳诱导的脂质过氧化过程中，大鼠肝脏全匀浆中的谷胱甘肽（GSH）含量在体外孵育的最初30分钟内迅速下降。在孵育期间，四氯化碳不会加剧总谷胱甘肽（TG）的减少，因此GSH的损失主要是由于其氧化为氧化型谷胱甘肽（GSSG）。相反，两种降低脂质过氧化速率的物质——磷脂酰甘油（PG）和乙二胺四乙酸（EDTA），能够减少GSH的氧化，而不影响TG含量。在25℃时，EDTA和PG在pH 7.4时能完全阻止GSH的减少，而在pH 6时PG只能部分阻止。在37℃时，这两种化合物都能在很大程度上限制GSH的减少。至少在体外，脂质过氧化在GSH减少和GSSG形成的动力学中似乎起着重要作用。值得注意的是，抑制四氯化碳刺激的脂质过氧化的PG，也能够限制在卤代烷存在下孵育的匀浆中观察到的大量GSH损失。