Risau W, Symmons P, Saumweber H, Frasch M
Cell. 1983 Jun;33(2):529-41. doi: 10.1016/0092-8674(83)90434-8.
Monoclonal antibodies have previously been raised against chromosomal proteins of Drosophila. Using a biochemical fractionation method for the isolation of large hnRNA-containing structures (hnRNP) of Drosophila tissue culture cells, we show that seven of these antibodies recognize different antigens, and that these antigens are associated with RNA. Analysis of the sedimentation behavior of antigen-containing structures in sucrose gradients reveals that the antigens are differentially distributed with respect both to one another and to pulse-labeled RNA. We demonstrate that the antigens are minor components of hnRNP and are different from the major Drosophila hnRNP packaging proteins, which we have also identified. The antigens are probably involved in the processing of hnRNA in the nucleus.
以前曾制备出针对果蝇染色体蛋白的单克隆抗体。利用一种生物化学分级分离方法来分离果蝇组织培养细胞中含大量核不均一RNA的结构（核不均一核糖核蛋白），我们发现其中七种抗体识别不同的抗原，并且这些抗原与RNA相关。对蔗糖梯度中含抗原结构的沉降行为分析表明，这些抗原彼此之间以及与脉冲标记的RNA的分布都存在差异。我们证明这些抗原是核不均一核糖核蛋白的次要成分，并且与我们已鉴定出的主要果蝇核不均一核糖核蛋白包装蛋白不同。这些抗原可能参与细胞核中核不均一RNA的加工过程。
