Hata N, Ito M, Mizuta H, Nose O, Miyai K
Clin Chem. 1983 Jul;29(7):1437-40.
A double-antibody enzyme immunoassay was developed for determination of thyroxin-binding globulin in dried blood samples on filter paper. The measurable concentration range of thyroxin-binding globulin in two 3-mm blood discs was 3.3 to 52 mg/L equivalent of serum (i.e., equivalent to the concentrations in known serum standards). Thyroxin-binding globulin in dried blood samples on filter paper was stable for at least four weeks when kept dry at -20 degrees C, 4 degrees C, or room temperature. The mean coefficients of variation were 6.6% (within assay) and 5.9% (between assays). The concentrations of thyroxin-binding globulin in dried blood samples determined by this method correlated well with those in serum determined by radioimmunoassay (r = 0.95) and by enzyme immunoassay (r = 0.96). This method is applicable for detecting cases of thyroxin-binding globulin deficiency and avoids the false-positive results for neonatal hypothyroidism obtained by measuring thyroxin.
建立了一种双抗体酶免疫测定法，用于测定滤纸上干血样中的甲状腺素结合球蛋白。在两片3毫米血片中，甲状腺素结合球蛋白的可测量浓度范围为相当于血清的3.3至52毫克/升（即相当于已知血清标准品中的浓度）。滤纸干血样中的甲状腺素结合球蛋白在-20℃、4℃或室温下干燥保存时，至少稳定四周。平均变异系数分别为6.6%（批内）和5.9%（批间）。用该方法测定的干血样中甲状腺素结合球蛋白的浓度与放射免疫测定法（r = 0.95）和酶免疫测定法（r = 0.96）测定的血清中浓度相关性良好。该方法适用于检测甲状腺素结合球蛋白缺乏症病例，避免了通过测定甲状腺素获得的新生儿甲状腺功能减退症的假阳性结果。
