Miyai K, Ito M, Hata N
Clin Chem. 1982 Dec;28(12):2408-11.
We describe a new double-antibody enzyme radioimmunoassay for thyroxin-binding globulin (TBG) in serum. A TBG-beta-D-galactosidase complex was prepared with m-maleimidobenzoyl N-hydroxysuccinimide ester. The mean analytical recovery of TBG added to serum was 99%, sample volume and TBG value were linearly related, and values for TBG determined by this method and those determined by radioimmunoassay correlated well (r = 0.98). Coefficients of variation averaged 7.6% within assay, 6.6% between assay. The measurable range of TBG in the serum was 3.3 to 52 mg/L, but higher TBG concentrations could be measured by diluting the test serum with TBG-free serum. Mean (and SD) serum TBG concentrations as determined by this method were as follows: for 20 normal subjects, 21.2 (3.7) mg/L; for 10 pregnant women, 50.1 (9.5 mg/L; and for 10 patients with TBG deficiency, not detectable.
我们描述了一种用于测定血清中甲状腺素结合球蛋白（TBG）的新型双抗体酶放射免疫分析法。用间马来酰亚胺苯甲酰N-羟基琥珀酰亚胺酯制备了TBG-β-D-半乳糖苷酶复合物。添加到血清中的TBG的平均分析回收率为99%，样本体积与TBG值呈线性相关，通过该方法测定的TBG值与通过放射免疫分析法测定的值相关性良好（r = 0.98）。批内变异系数平均为7.6%，批间变异系数为6.6%。血清中TBG的可测量范围为3.3至52 mg/L，但通过用无TBG血清稀释测试血清可以测量更高的TBG浓度。通过该方法测定的平均（及标准差）血清TBG浓度如下：20名正常受试者为21.2（3.7）mg/L；10名孕妇为50.1（9.5）mg/L；10名TBG缺乏患者未检测到。
