Hotchin J, Sikora E, Baker F
Intervirology. 1983;19(4):205-12. doi: 10.1159/000149362.
Examination of newborn mice, inoculated intraperitoneally with high doses of scrapie virus, revealed that the virus could not be reisolated from their tissues after about 1 week following inoculation, until almost 1 year later. The inoculum was rapidly removed and was not detectable, although the animals became latently infected. Homogenization of whole inoculated newborn animals showed that only about 3% of virus could be recovered by the 2nd day postinoculation (p.i.). During the first 6 days p.i. the half-life of titratable scrapie was about 15 h. A further study of the rate of disappearance of clarified scrapie virus from blood after intravenous inoculation showed an even more rapid disappearance, with a half-life of 5.16 min. Prior treatment of the recipient mice with either carbon black or silica to block the reticuloendothelial system (RES) did not affect the rate of disappearance. It was concluded that the mouse possesses a very efficient means of scrapie virus removal from the blood which is not dependent upon an active RES. However, after 1 h the rate of disappearance changed dramatically; the residual virus level was very stable, with no significant drop during the next 21 h. This finding was compatible with the possibility that two forms of scrapie virus, with different removal rates, coexisted in the inoculum. Silica treatment caused a shortened scrapie incubation period.
对经腹腔接种高剂量羊瘙痒病病毒的新生小鼠进行检查发现，接种后约1周内，直至将近1年后，均无法从其组织中重新分离出该病毒。尽管动物已被潜伏感染，但接种物迅速被清除且无法检测到。对接种后的新生动物整体进行匀浆处理显示，接种后第2天仅能回收约3%的病毒。在接种后的前6天，可滴定羊瘙痒病病毒的半衰期约为15小时。另一项关于静脉接种后血液中澄清羊瘙痒病病毒消失速率的研究显示，其消失速度更快，半衰期为5.16分钟。预先用炭黑或二氧化硅处理受体小鼠以阻断网状内皮系统（RES），并不影响病毒消失的速率。由此得出结论，小鼠拥有一种非常有效的从血液中清除羊瘙痒病病毒的方式，且该方式不依赖于活跃的RES。然而，1小时后消失速率发生了显著变化；残留病毒水平非常稳定，在接下来的21小时内没有明显下降。这一发现与接种物中存在两种清除速率不同的羊瘙痒病病毒形式的可能性相符。二氧化硅处理导致羊瘙痒病潜伏期缩短。
