脊椎动物神经肌肉接头的细胞骨架成分：纽蛋白、α-辅肌动蛋白和细丝蛋白。
Cytoskeletal components of the vertebrate neuromuscular junction: vinculin, alpha-actinin, and filamin.
作者信息
Bloch R J, Hall Z W
出版信息
J Cell Biol. 1983 Jul;97(1):217-23. doi: 10.1083/jcb.97.1.217.
Abstract
We have used immunocytochemical methods to investigate the cytoskeletal constituents of the vertebrate neuromuscular junction. Specific, affinity-purified antibodies to three cytoskeletal proteins, vinculin, alpha-actinin, and filamin, bound to neuromuscular junctions in sections of normal rat, mouse, chick, and Xenopus muscles. All three antibodies bound to the synaptic regions of denervated rat muscle fibers, indicating that the proteins recognized by these antibodies are associated with postsynaptic structures. The three proteins are present at the neuromuscular junction in muscle fibers of embryonic and neonatal animals, and therefore, may play an important role in its differentiation.
摘要
我们运用免疫细胞化学方法研究了脊椎动物神经肌肉接头的细胞骨架成分。针对三种细胞骨架蛋白（纽蛋白、α-辅肌动蛋白和细丝蛋白）的特异性亲和纯化抗体，能与正常大鼠、小鼠、鸡和非洲爪蟾肌肉切片中的神经肌肉接头结合。所有这三种抗体都与去神经支配的大鼠肌纤维的突触区域结合，表明这些抗体识别的蛋白与突触后结构相关。这三种蛋白存在于胚胎和新生动物肌纤维的神经肌肉接头处，因此可能在其分化过程中发挥重要作用。
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Immunofluorescence localization at the mammalian neuromuscular junction of the Mr 43,000 protein of Torpedo postsynaptic membranes.电鳐突触后膜43000道尔顿蛋白质在哺乳动物神经肌肉接头处的免疫荧光定位。
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Association of fibronectin and vinculin with focal contacts and stress fibers in stationary hamster fibroblasts.纤连蛋白和纽蛋白与静止仓鼠成纤维细胞中粘着斑和应力纤维的关联
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