Bolton F J, Coates D
J Clin Pathol. 1983 Jul;36(7):829-34. doi: 10.1136/jcp.36.7.829.
The oxygen and carbon dioxide requirements of different biotypes of thermophilic campylobacters were investigated by means of (a) quantitative studies, and (b) total growth studies. Oxygen tolerance of the five test organisms differed markedly and varied with the carbon dioxide concentration. At most carbon dioxide concentrations tested, Campylobacter jejuni strains NCTC 11168 and NCTC 11392 tolerated 21% oxygen (growth reduced), C coli NCTC 11353 tolerated 15% oxygen (growth reduced), and C jejuni ATCC 3036 and (nalidixic acid resistant thermophilic campylobacter) NCTC 11352 tolerated 10% oxygen (growth not reduced). Total growth studies indicated that 10% oxygen was the optimal concentration for growth of the five test organisms. All exhibited a requirement for carbon dioxide, and only C jejuni strains NCTC 11168 and NCTC 11392 tolerated its absence (growth reduced), when the oxygen concentration was low. The studies indicated that atmospheres containing 5% to 10% oxygen and 1.0% to 10% carbon dioxide are suitable for growth of the various biotypes of thermophilic campylobacters. The oxygen and carbon dioxide concentrations produced in anaerobic jars by variations of the evacuation-replacement technique were determined and suitable practices identified.
通过（a）定量研究和（b）总生长研究，对嗜热弯曲杆菌不同生物型的氧气和二氧化碳需求进行了调查。五种受试微生物的耐氧性差异显著，且随二氧化碳浓度而变化。在大多数测试的二氧化碳浓度下，空肠弯曲杆菌菌株NCTC 11168和NCTC 11392能耐受21%的氧气（生长减少），结肠弯曲杆菌NCTC 11353能耐受15%的氧气（生长减少），空肠弯曲杆菌ATCC 3036和（耐萘啶酸嗜热弯曲杆菌）NCTC 11352能耐受10%的氧气（生长未减少）。总生长研究表明，10%的氧气浓度是五种受试微生物生长的最佳浓度。所有菌株都表现出对二氧化碳的需求，当氧气浓度较低时，只有空肠弯曲杆菌菌株NCTC 11168和NCTC 11392能耐受无二氧化碳环境（生长减少）。研究表明，含有5%至10%氧气和1.0%至10%二氧化碳的气体环境适合嗜热弯曲杆菌各种生物型的生长。测定了通过抽空-置换技术变化在厌氧罐中产生的氧气和二氧化碳浓度，并确定了合适的操作方法。
