Cramer E B, Pologe L, Pawlowski N A, Cohn Z A, Scott W A
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4109-13. doi: 10.1073/pnas.80.13.4109.
Cultured endothelial cells from human umbilical vein were labelled with [3H]arachidonic acid for 16 hr. The radiolabel was localized primarily in phospholipids (93%) and 73% was distributed equally between phosphatidylcholine and phosphatidylethanolamine. Leukotriene C (10-1,000 nM) promoted a dose-dependent release of radiolabel into the culture medium. This response was 3.3 times control values at 100 nM. The major arachidonic acid metabolite synthesized was prostacyclin, which was 33% of the total released radiolabel. Endothelial cells also released small amounts of prostaglandin F2 alpha (6.1%), unidentified lipoxygenase products (14.8%), and unreacted arachidonic acid (33%). The 30-min time course of release was independent of the leukotriene C concentration used. Leukotriene D at similar concentrations also promoted endothelial cells to release primarily prostacyclin and unreacted arachidonic acid. The release of prostacyclin, a potent vasodilator agent, may be an important mediator in slow reacting substance effects on the vasculature.
用人脐静脉培养的内皮细胞用[3H]花生四烯酸标记16小时。放射性标记主要定位于磷脂（93%），且73%在磷脂酰胆碱和磷脂酰乙醇胺之间平均分布。白三烯C（10 - 1000 nM）促使放射性标记呈剂量依赖性释放到培养基中。在100 nM时，该反应是对照值的3.3倍。合成的主要花生四烯酸代谢产物是前列环素，占总释放放射性标记的33%。内皮细胞还释放少量前列腺素F2α（6.1%）、未鉴定的脂氧合酶产物（14.8%）和未反应的花生四烯酸（33%）。30分钟的释放时间进程与所用白三烯C的浓度无关。相似浓度的白三烯D也促使内皮细胞主要释放前列环素和未反应的花生四烯酸。前列环素作为一种强效血管舒张剂，其释放可能是慢反应物质对脉管系统作用的重要介质。
