Onodera H, Takada G, Tada K, Desnick R J
Tohoku J Exp Med. 1983 May;140(1):1-13. doi: 10.1620/tjem.140.1.
The localization of intravenously injected liposome-entrapped or unentrapped 3H- beta-ga lactosidase in various tissues of rats was investigated by microautoradiography. The microautoradiographic silver grains, indicating the uptake of both forms of the enzyme were observed in all of the rat tissues studied, such as the liver, kidneys, spleen, lungs, heart, muscle and brain. The liver was found to be the most active in taking up both forms of the enzyme and probably Kupffer cells were primarily involved in the uptake of the enzyme. Also in the brain, the uptake of both forms of the enzyme was observed, though far less extensively, through the blood-brain barrier. The uptake of the enzyme in the brain was somewhat greater in the gray matter than in the white matter.
通过显微放射自显影术研究了静脉注射的脂质体包裹或未包裹的3H-β-半乳糖苷酶在大鼠各种组织中的定位。在所有研究的大鼠组织中，如肝脏、肾脏、脾脏、肺、心脏、肌肉和大脑，都观察到了显微放射自显影银颗粒，表明两种形式的酶都被摄取。发现肝脏摄取两种形式的酶最为活跃，库普弗细胞可能主要参与了酶的摄取。在大脑中，也观察到两种形式的酶通过血脑屏障被摄取，尽管摄取程度远低于肝脏。大脑灰质中酶的摄取量比白质中略高。
