布鲁氏菌流产抗原诱导豚鼠淋巴细胞反应性

Brucella abortus antigen-induced lymphocyte reactivity in guinea pigs.

作者信息

Jones R L, Buening G M

出版信息

Vet Immunol Immunopathol. 1983 May;4(4):433-43. doi: 10.1016/0165-2427(83)90003-x.

Abstract

A whole blood lymphocyte transformation (WBLT) assay was used to detect anti-brucella lymphocyte reactivity in guinea pigs. Brucella antigens stimulated an antigen-specific lymphoproliferative response in WBLT assays from Brucella abortus infected guinea pigs. The response was best detected from 6 to 16 weeks after challenge inoculation with viable B. abortus 2308. Lymphocytes were not stimulated by unrelated bacterial antigens and control animals did not respond to the Brucella antigens. The responding cell population was characterized as mostly T lymphocytes. The WBLT assay was found to be specific for the detection of anti-brucella lymphocyte reactivity. However, a negative response was not definitive, which indicated a need for repeated testing to establish that a guinea pig did not have anti-brucella lymphocyte sensitization.

摘要

采用全血淋巴细胞转化（WBLT）试验检测豚鼠抗布鲁氏菌淋巴细胞反应性。在流产布鲁氏菌感染豚鼠的WBLT试验中，布鲁氏菌抗原刺激产生了抗原特异性淋巴细胞增殖反应。在用活的流产布鲁氏菌2308进行攻击接种后6至16周时，该反应检测效果最佳。无关细菌抗原未刺激淋巴细胞，对照动物对布鲁氏菌抗原无反应。应答细胞群体主要为T淋巴细胞。发现WBLT试验对检测抗布鲁氏菌淋巴细胞反应性具有特异性。然而，阴性反应并不确定，这表明需要重复检测以确定豚鼠是否没有抗布鲁氏菌淋巴细胞致敏。