Ligler F S, Kettman J R, Smith R G, Frenkel E P
Blood. 1983 Aug;62(2):256-63.
The present study examines the relative amounts of surface immunoglobulin (Ig) on lymphocytes obtained from 64 patients with chronic lymphocytic leukemia (CLL) and correlates these findings with the clinical stage of disease. Since the maturing B cell first expresses surface IgM, followed by IgD, and subsequently by IgG, IgA, or IgE, the surface Ig phenotype can be used as a marker of differentiation. Surface Ig was analyzed using the fluorescence-activated cell sorter under carefully controlled conditions. The cells from all patients with CLL were monoclonal with respect to light chain type. Those patients with IgM as the brightest heavy chain class (suggesting relatively immature B cells) had clinically advanced stages of CLL, whereas those with a predominance of IgG (suggesting more mature cells) have a lesser stage of CLL. Thus, the predominant Ig heavy chain class appears to correlate with clinical stage of CLL and provides a clue to the potential aggressiveness of the tumor.
本研究检测了64例慢性淋巴细胞白血病（CLL）患者淋巴细胞表面免疫球蛋白（Ig）的相对含量，并将这些结果与疾病的临床分期相关联。由于成熟的B细胞首先表达表面IgM，随后是IgD，接着是IgG、IgA或IgE，因此表面Ig表型可作为分化的标志物。在严格控制的条件下，使用荧光激活细胞分选仪分析表面Ig。所有CLL患者的细胞在轻链类型方面均为单克隆。那些以IgM作为最亮重链类别（提示相对不成熟的B细胞）的患者，CLL处于临床晚期，而那些以IgG为主（提示细胞更成熟）的患者，CLL分期较低。因此，主要的Ig重链类别似乎与CLL的临床分期相关，并为肿瘤的潜在侵袭性提供了线索。
