Wiesel J M, Gamiel H, Vlodavsky I, Gay I, Ben-Bassat H
Eur J Clin Invest. 1983 Feb;13(1):57-63. doi: 10.1111/j.1365-2362.1983.tb00065.x.
The advantageous utilization of nasal polypoid tissue as a source for human epithelial cells and the dramatic effect of extracellular matrix on growth and differentiation of these cells were demonstrated. Plating on extracellular matrix induced rapid, firm, cell attachment and flattening of the explants, promoted cell outgrowth as well as long-term survival of epithelial cells in primary cultures. Prominent ciliary activity was observed on the cells of the explant and on the cells in the outgrowth. These cells could be maintained on the extracellular matrix coated dishes for prolonged periods even after removal of the explant, with the cells in the outgrowth covering the region occupied by the removed explant. Prominent ciliary activity, which is considered one of the main criteria for cell viability and differentiation, continued also in the absence of the explant. The present system for cultivation of human upper respiratory tract epithelial cells on extracellular matrix might prove of value in analysing effects of chemotherapeutic agents that influence normal differentiation as well as the effects of viral and chemical carcinogens on these cells in human respiratory disease.
已证实鼻息肉样组织作为人类上皮细胞来源的有利利用以及细胞外基质对这些细胞生长和分化的显著影响。接种于细胞外基质可诱导外植体快速、牢固地附着并使细胞扁平，促进细胞生长以及原代培养上皮细胞的长期存活。在外植体的细胞和生长物中的细胞上观察到明显的纤毛活动。即使在移除外植体后，这些细胞仍可在细胞外基质包被的培养皿上长时间维持，生长物中的细胞覆盖了被移除的外植体所占据的区域。在没有外植体的情况下，作为细胞活力和分化主要标准之一的明显纤毛活动也持续存在。目前在细胞外基质上培养人上呼吸道上皮细胞的系统可能在分析影响正常分化的化疗药物的作用以及病毒和化学致癌物对人类呼吸道疾病中这些细胞的作用方面具有价值。
