Almagor M, Kahane I, Wiesel J M, Yatziv S
Infect Immun. 1985 May;48(2):552-5. doi: 10.1128/iai.48.2.552-555.1985.
Human ciliated epithelial cells derived from nasal polyps and cultured in a monolayer were studied as an experimental model for Mycoplasma pneumoniae infection. Scanning electron microscopy revealed two types of cultured epithelial cells: one which was covered by microvilli only and another which had microvilli and actively beating cilia. M. pneumoniae adhered to both types of cells, and the adherence followed saturation kinetics as a function of time. Infection of the cells for 20 h resulted in 75% inhibition of their intracellular catalase activity and a 3.5-fold increase in their malonyldialdehyde levels compared with noninfected controls. This indicates the presence of cellular oxidative damage due to M. pneumoniae infection. It is suggested that human nasal ciliated epithelial cells may serve as a representative model for studying M. pneumoniae in relation to its natural host.
将来自鼻息肉并单层培养的人纤毛上皮细胞作为肺炎支原体感染的实验模型进行研究。扫描电子显微镜显示出两种培养的上皮细胞：一种仅覆盖有微绒毛，另一种既有微绒毛又有活跃跳动的纤毛。肺炎支原体附着于这两种类型的细胞，并且附着遵循作为时间函数的饱和动力学。与未感染的对照相比，细胞感染20小时导致其细胞内过氧化氢酶活性受到75%的抑制，丙二醛水平增加3.5倍。这表明存在由肺炎支原体感染引起的细胞氧化损伤。有人提出，人鼻纤毛上皮细胞可作为研究肺炎支原体与其天然宿主关系的代表性模型。
