Zahm J M, Lamiot E, Pierrot D, Chevillard M, Hinnrasky J, Puchelle E
INSERM U.314, Université de Reims, France.
In Vitro Cell Dev Biol. 1990 Nov;26(11):1063-7. doi: 10.1007/BF02624441.
Ciliated cell cultures can be produced in outgrowths from explants of human respiratory epithelium. An image analysis technique was developed to quantify the percentage of active ciliated cells present in these cultures. The subtraction 2 by 2 of five successive video images of the cultures, followed by the addition of the resulting images, allowed the determination of the culture surface covered by ciliated cells. The percentage of this surface varied according to the region of the explant (27.7% in the outgrowth near the explant and 4.1% at the periphery of the outgrowth). High variations were observed within the same region of an outgrowth, as well as from one outgrowth to another. However, maximal differentiation was observed after 4 d of culture. The quantitation technique described in the present work might be useful for studying in vitro the respiratory epithelial injury and the subsequent repair processes.
纤毛细胞培养物可从人呼吸道上皮外植体的生长物中产生。开发了一种图像分析技术来量化这些培养物中活性纤毛细胞的百分比。对培养物的五个连续视频图像进行两两相减，然后将所得图像相加，从而确定纤毛细胞覆盖的培养物表面。该表面的百分比根据外植体的区域而变化（在外植体附近的生长物中为27.7%，在生长物的周边为4.1%）。在同一个生长物的同一区域内以及从一个生长物到另一个生长物之间都观察到了很大的差异。然而，在培养4天后观察到最大程度的分化。本研究中描述的定量技术可能有助于在体外研究呼吸道上皮损伤及随后的修复过程。
