Costello G T, Heppe R, Winn H R, Scheld W M, Rodeheaver G T
Infect Immun. 1983 Aug;41(2):535-9. doi: 10.1128/iai.41.2.535-539.1983.
The utility of an experimental animal model is dependent on its ability to simulate the actual clinical situation. With a stereotaxic injection procedure, the susceptibility of rat brain to the spectrum of organisms commonly associated with human brain abscess was determined. Two strains of Escherichia coli were more infective than Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pyogenes. Even between the E. coli strains it was possible to document significant differences in degree of infectivity. The E. coli strain with the K-1 capsular polysaccharide was significantly more infective than the E. coli strain without the capsular polysaccharide. The brain was also susceptible to Candida albicans, but at a level higher than any of the aerobic bacteria examined. Brain infection could not be created when microaerophilic or obligately anaerobic organisms alone were injected.
实验动物模型的效用取决于其模拟实际临床情况的能力。通过立体定向注射程序，确定了大鼠脑对通常与人类脑脓肿相关的一系列微生物的易感性。两株大肠杆菌比铜绿假单胞菌、金黄色葡萄球菌和化脓性链球菌更具感染性。即使在大肠杆菌菌株之间，也能够证明感染程度存在显著差异。具有K-1荚膜多糖的大肠杆菌菌株比没有荚膜多糖的大肠杆菌菌株感染性明显更强。脑对白色念珠菌也易感，但程度高于所检测的任何需氧菌。单独注射微需氧菌或专性厌氧菌时无法造成脑感染。
