Kannangara D W, Thadepalli H, Bach V T, Webb D
Infect Immun. 1981 Feb;31(2):592-7. doi: 10.1128/iai.31.2.592-597.1981.
There are no satisfactory animal models for the study of anaerobic lung abscess. Aspiration of food, gastric mucin, or hydrochloric acid, or any combination of these, along with oropharyngeal bacteria, is commonly believed to cause aspiration pneumonia and lung abscess. In the animal model described, none of the adjuvants was effective in producing anaerobic lung abscesses. Anaerobic bacteria derived from dental scrapings of a healthy adult (Peptococcus morbillorum, Fusobacterium nucleatum, Eubacterium lentum, and Bacteroides fragilis), when inoculated transtracheally without any adjuvants into New Zealand male white rabbits, consistently produced lung abscesses. Neither B fragilis by itself nor a mixture of P. morbillorum, F. nucleatum, and E. lentum without the addition of B. fragilis produced lung abscesses. The bacterial isolates used in this study were stored in prereduced chopped-meat-glucose medium and subcultured several times and were found effective in reproducing anaerobic lung abscesses repeatedly. This animal model is suitable for the study of pathogenesis, diagnosis, and treatment of B. fragilis-associated anaerobic lung abscess.
目前尚无用于研究厌氧性肺脓肿的理想动物模型。通常认为，吸入食物、胃黏液或盐酸，或这些物质的任何组合，再加上口咽细菌，会导致吸入性肺炎和肺脓肿。在所描述的动物模型中，没有一种佐剂能有效引发厌氧性肺脓肿。从一名健康成年人的牙菌斑中分离出的厌氧细菌（麻疹消化球菌、具核梭杆菌、迟缓真杆菌和脆弱拟杆菌），在不添加任何佐剂的情况下经气管接种到新西兰雄性白兔体内，始终会引发肺脓肿。单独的脆弱拟杆菌或不添加脆弱拟杆菌的麻疹消化球菌、具核梭杆菌和迟缓真杆菌的混合物均不会引发肺脓肿。本研究中使用的细菌分离株保存在预先还原的碎肉葡萄糖培养基中，并多次传代培养，发现其能有效反复重现厌氧性肺脓肿。该动物模型适用于研究与脆弱拟杆菌相关的厌氧性肺脓肿的发病机制、诊断和治疗。
