需氧和厌氧多形核中性粒细胞的杀菌活性。
Bactericidal activity of aerobic and anaerobic polymorphonuclear neutrophils.
作者信息
Mandell G L
出版信息
Infect Immun. 1974 Feb;9(2):337-41. doi: 10.1128/iai.9.2.337-341.1974.
Abstract
Human polymorphonuclear neutrophils (PMN) were made anaerobic by nitrogen washout (oxygen saturation <1%, Eh < -42 mV at pH 7.0), and the ability of the cells to kill bacteria was assayed and compared to the bactericidal activity of aerobic PMN. Anaerobic PMN were able to kill Staphylococcus epidermidis, Enterococcus, viridans streptococci, Pseudomonas aeruginosa, Peptostreptococcus anaerobius, Bacteroides fragilis, Clostridium perfringens, and Peptococcus magnus normally. Organisms that were not killed normally by anaerobic PMN included Staphylococcus aureus (strains Wood 46 and 502a), Escherichia coli, Serratia marcescens, Klebsiella pneumoniae, Proteus vulgaris, and Salmonella typhimurium. These findings suggest that mechanisms other than those dependent on hydrogen peroxide may be important in the killing of some bacteria.
摘要
通过氮气冲洗使人类多形核中性粒细胞（PMN）处于厌氧状态（氧饱和度<1%，pH 7.0时氧化还原电位< -42 mV），然后检测细胞杀灭细菌的能力，并与需氧PMN的杀菌活性进行比较。厌氧PMN能够正常杀灭表皮葡萄球菌、肠球菌、草绿色链球菌、铜绿假单胞菌、厌氧消化链球菌、脆弱拟杆菌、产气荚膜梭菌和大消化球菌。厌氧PMN不能正常杀灭的微生物包括金黄色葡萄球菌（伍德46株和502a株）、大肠杆菌、粘质沙雷氏菌、肺炎克雷伯菌、普通变形杆菌和鼠伤寒沙门氏菌。这些发现表明，除了依赖过氧化氢的机制外，其他机制可能在某些细菌的杀灭中起重要作用。
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