Beuvery E C, van Delft R W, Miedema F, Kanhai V, Nagel J
Infect Immun. 1983 Aug;41(2):609-17. doi: 10.1128/iai.41.2.609-617.1983.
Neisseria meningitidis group C polysaccharide-tetanus toxoid conjugate was prepared to obtain the polysaccharide component in a thymus-dependent form and to preserve the immunogenic properties of the tetanus toxoid component. Biochemical and immunochemical analyses of this conjugate revealed that (i) it was composed of equal amounts of polysaccharide and protein; (ii) the antigenic activity of the polysaccharide component was greatly reduced; (iii) it contained about 10% free polysaccharide; (iv) the composition was not homogeneous; and (v) only 5% of the tetanus toxoid component was present at the surface of the conjugate molecules. In this study, the influence of these characteristics on the antibody response to both components in mice was investigated. The dose-response relationship, the persistence of antibodies, a possible antigenic competition, and the specificities of the antibodies induced were also studied. Our data suggest that the conjugate behaves as a pronounced thymus-dependent antigen, that the tetanus toxoid component is more immunogenic at lower dosages (0.8 and 20 ng) than equivalent doses of tetanus vaccine, that the presence of free polysaccharide does not influence the induction of antibodies to polysaccharide by the conjugate, and that no antibodies to new structures in the conjugate are induced. These characteristics favor the application of this conjugate as a vaccine for human use.
制备了C群脑膜炎奈瑟菌多糖-破伤风类毒素结合物，以获得胸腺依赖性形式的多糖成分，并保留破伤风类毒素成分的免疫原性。对该结合物的生化和免疫化学分析表明：（i）它由等量的多糖和蛋白质组成；（ii）多糖成分的抗原活性大大降低；（iii）它含有约10%的游离多糖；（iv）组成不均一；（v）仅5%的破伤风类毒素成分存在于结合物分子表面。在本研究中，研究了这些特性对小鼠体内针对两种成分的抗体反应的影响。还研究了剂量反应关系、抗体的持久性、可能的抗原竞争以及诱导抗体的特异性。我们的数据表明，该结合物表现为一种明显的胸腺依赖性抗原，破伤风类毒素成分在较低剂量（0.8和20 ng）时比等量的破伤风疫苗更具免疫原性，游离多糖的存在不影响结合物诱导针对多糖的抗体，并且不会诱导针对结合物中新结构的抗体。这些特性有利于将该结合物用作人用疫苗。
