C群脑膜炎球菌多糖-破伤风类毒素结合物作为新一代疫苗的制备及免疫化学特性分析
Preparation and immunochemical characterization of meningococcal group C polysaccharide-tetanus toxoid conjugates as a new generation of vaccines.
作者信息
Beuvery E C, Miedema F, van Delft R, Haverkamp J
出版信息
Infect Immun. 1983 Apr;40(1):39-45. doi: 10.1128/iai.40.1.39-45.1983.
Abstract
Neisseria meningitidis group C polysaccharide-tetanus toxoid conjugates have been prepared by using high-molecular-weight polysaccharide and purified tetanus toxoid and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide as a coupling reagent. The influence of three conditions of preparation was studied. Biochemical assays, the enzyme-linked immunosorbent assay, and isopycnic CsCl gradient ultracentrifugation have been used to characterize the conjugates. The polysaccharide-to-protein ratios of the various conjugate preparations showed differences. The ability of the group C polysaccharide component to react with specific antibodies was reduced, whereas most of the tetanus toxoid seemed to be hidden by the polysaccharide. The composition of the conjugate was not homogeneous, and at least 10% of free polysaccharide was present. Thermostability in lyophilized condition in the presence of lactose was excellent.
摘要
C群脑膜炎奈瑟菌多糖-破伤风类毒素结合物是通过使用高分子量多糖、纯化的破伤风类毒素以及1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺作为偶联剂制备而成的。研究了三种制备条件的影响。采用生化分析、酶联免疫吸附测定和等密度氯化铯梯度超速离心法对结合物进行表征。各种结合物制剂的多糖与蛋白质比例存在差异。C群多糖成分与特异性抗体反应的能力降低，而大部分破伤风类毒素似乎被多糖掩盖。结合物的组成不均匀，至少存在10%的游离多糖。在乳糖存在下冻干条件下的热稳定性极佳。
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