Isacsson G, Hammarström L
J Oral Pathol. 1983 Jun;12(3):217-22. doi: 10.1111/j.1600-0714.1983.tb00335.x.
An investigation was undertaken to study the distribution of enzymes associated with submandibular gland salivary calculi. Ten calculi were freeze-sectioned and incubated for acid and alkaline phosphatases and for lactate, succinate and maleate dehydrogenases. All calculi were partly covered by a 50-210 micrometers wide zone of organic material consisting of connective tissue and metaplastic squamous epithelium facing the mineralized calculus, or of a structureless substance attached to the mineralized calculus. The epithelium showed an intense staining reaction for acid phosphatase and lactate dehydrogenase and a moderate reaction for succinate dehydrogenase throughout all levels of the epithelium. The structureless peripheral zone exhibited a moderate activity of acid phosphatase and succinate dehydrogenase located to an area close to the mineralized matrix. Also alkaline phosphatase and lactate dehydrogenase were found in a special pattern in the structureless zone. Sodium fluoride and sodium vanadate added to the incubation medium inhibited acid phosphatase activity whereas cupric chloride only lowered the staining reaction. Enzyme activity was found only within the peripheral zone of organic material with one exception. The results suggest that the calcification process of salivary calculi is not a passive calcification of necrotic material or mucin but rather an active process promoted by enzymes in the surrounding organic substances.
开展了一项研究，以探究与下颌下腺涎石相关的酶的分布情况。对10颗结石进行冷冻切片，并针对酸性和碱性磷酸酶以及乳酸、琥珀酸和马来酸脱氢酶进行孵育。所有结石部分被一个宽50 - 210微米的有机物质区域覆盖，该区域由结缔组织和面向矿化结石的化生鳞状上皮组成，或者由附着在矿化结石上的无结构物质组成。上皮在整个上皮各层对酸性磷酸酶和乳酸脱氢酶呈现强烈染色反应，对琥珀酸脱氢酶呈现中度反应。无结构的周边区域在靠近矿化基质的区域呈现出中度的酸性磷酸酶和琥珀酸脱氢酶活性。在无结构区域还发现碱性磷酸酶和乳酸脱氢酶呈现特殊模式。添加到孵育培养基中的氟化钠和钒酸钠抑制酸性磷酸酶活性，而氯化铜仅降低染色反应。除一个例外情况外，酶活性仅在有机物质的周边区域被发现。结果表明，涎石的钙化过程并非坏死物质或粘蛋白的被动钙化，而是由周围有机物质中的酶促进的一个主动过程。
