Friskopp J, Hammarström L
Acta Odontol Scand. 1982;40(6):459-66. doi: 10.3109/00016358209025121.
The following investigation was undertaken to study the location in the dental plaque and calculus of certain enzyme activities and to compare the patterns obtained with those of the normal hard tissue formation. Supragingival and subgingival calculus attached to the root surfaces of 30 extracted teeth was studied. The root with its deposits was frozen rapidly in a mixture of hexane and solid CO2 (-75 degrees C). From the frozen block, sections were cut and incubated for histochemical demonstration of lactate dehydrogenase, alkaline phosphatase and acid phosphatase. The plaque seemed to be stratified with regard to enzyme activity. Three different layers could be identified. In the basal layer, approximately 100 microns thick, enzyme activity was low. Lactate dehydrogenase activity could be identified in some sections, but no phosphatase activity. In the middle layer lactate dehydrogenase, alkaline phosphatase and acid phosphatase activities were found in most of the sections. The superficial layer usually showed lactate dehydrogenase but not always acid or alkaline phosphatase activities. The results of the present investigation may suggest that the mineralization of the dental plaque is not only a passive mineralization of dead bacteria, but also an active process promoted by enzymes in the covering bacterial layers.
进行以下研究以探讨某些酶活性在牙菌斑和牙结石中的定位，并将所得模式与正常硬组织形成的模式进行比较。研究了附着在30颗拔除牙齿根面上的龈上和龈下牙结石。将带有沉积物的牙根迅速冷冻在己烷和固态二氧化碳的混合物中（-75℃）。从冷冻块上切取切片，并进行孵育，以进行乳酸脱氢酶、碱性磷酸酶和酸性磷酸酶的组织化学显示。牙菌斑在酶活性方面似乎呈分层状。可识别出三个不同的层。在约100微米厚的基底层中，酶活性较低。在一些切片中可识别出乳酸脱氢酶活性，但无磷酸酶活性。在中间层，大多数切片中都发现了乳酸脱氢酶、碱性磷酸酶和酸性磷酸酶活性。表层通常显示乳酸脱氢酶活性，但并非总是显示酸性或碱性磷酸酶活性。本研究结果可能表明，牙菌斑的矿化不仅是死细菌的被动矿化，而且是覆盖细菌层中酶促进的一个活跃过程。
