Goya R G, Sosa Y E, Gómez C J
J Natl Cancer Inst. 1983 Aug;71(2):331-4.
A serial biopsy method was developed to study DNA synthesis in 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors during the regression process induced by bromocryptine (CB-154) administration in highly inbred female SD rats. With this technique the changes in tumor size could be correlated with those of DNA synthesis in single regressing tumors. DNA synthesis was estimated by the in vitro incorporation of tritiated thymidine into DNA which correlated well (correlation coefficient r = 0.95) with the in vivo mitotic activity of these neoplasms. Neither the biopsies themselves nor the estral status of the hosts affected significantly the rate of tumor DNA synthesis. DNA synthesis decreased sharply 4-8 days after the beginning of CB-154 treatment, whereas tumor shrinkage occurred more gradually. In conclusion, the serial biopsy method is a reliable technique for the estimation of changes in DNA synthesis in regressing DMBA-induced rat mammary tumors, whereas the measurement of the rate of tumor shrinkage is not.
我们开发了一种连续活检方法，用于研究在高度近交的雌性SD大鼠中，给予溴隐亭（CB - 154）诱导7,12 - 二甲基苯并[a]蒽（DMBA）诱导的乳腺肿瘤消退过程中的DNA合成情况。通过这种技术，肿瘤大小的变化可以与单个消退肿瘤中的DNA合成变化相关联。通过体外将氚标记的胸腺嘧啶核苷掺入DNA来估计DNA合成，这与这些肿瘤的体内有丝分裂活性相关性良好（相关系数r = 0.95）。活检本身以及宿主的动情状态均未显著影响肿瘤DNA合成速率。在开始CB - 154治疗后4 - 8天，DNA合成急剧下降，而肿瘤缩小则更为缓慢。总之，连续活检方法是评估DMBA诱导的大鼠乳腺肿瘤消退过程中DNA合成变化的可靠技术，而测量肿瘤缩小速率则不然。
