Résibois A, Toussaint A, de Smet L, Lefèbvre N
Gene. 1978 Sep;4(1):51-68. doi: 10.1016/0378-1119(78)90014-8.
We have isolated and characterized a mutant of temperate phage Mu-1 carrying an IS2 insertion in the middle of its beta region. This mutant gives rise spontaneously to secondary mutants which have deletions of different sizes adjacent to IS2. One particular derivative however, was found to have acquired an additional insertion sequence adjacent to IS2. This derivative gave rise to tertiary mutants carrying a deletion next to the tandem insertion. The tandem insertion was located at the same place in the Mu beta region as another 2.6 kb insertion independently isolated by Chow et al. (1977) and was found to be homologous to that insertion. The properties of this particular secondary mutant show that Mu phage particles lacking their S end are defective for growth and lysogenisation.
我们分离并鉴定了一种温和噬菌体Mu-1的突变体，该突变体在其β区域中部携带一个IS2插入序列。这种突变体自发产生二级突变体，这些二级突变体在IS2附近有不同大小的缺失。然而，发现一个特定的衍生物在IS2附近获得了一个额外的插入序列。这个衍生物产生了三级突变体，其在串联插入旁边携带一个缺失。该串联插入位于Mu β区域中与Chow等人（1977年）独立分离的另一个2.6 kb插入相同的位置，并且发现与该插入同源。这个特定二级突变体的特性表明，缺乏S末端的Mu噬菌体颗粒在生长和溶原化方面存在缺陷。
