Petrullo L A, Gallagher P J, Elseviers D
Mol Gen Genet. 1983;190(2):289-94. doi: 10.1007/BF00330653.
Readthrough and suppression of nonsense codons was compared in Escherichia coli strains with and without a miaA mutation, which confers a loss of the isopentenyladenosine modification in transfer RNA. Generally speaking, our results conform to predictions based on previous literature. In addition, we showed that the miaA mutation in strain TRPX is itself a UAA mutation. An antagonism between miaA and rpsL mutations, which confer streptomycin resistance, was also discovered. Our data further suggest that slight alterations of the translation apparatus are easily detectable by monitoring readthrough and suppression of nonsense codons. Our findings are discussed in the context of old and recent reports.
在具有和不具有miaA突变的大肠杆菌菌株中比较了通读和无义密码子抑制情况，miaA突变会导致转运RNA中异戊烯基腺苷修饰缺失。一般来说，我们的结果符合基于先前文献的预测。此外，我们表明菌株TRPX中的miaA突变本身就是一个UAA突变。还发现了miaA与赋予链霉素抗性的rpsL突变之间的拮抗作用。我们的数据进一步表明，通过监测通读和无义密码子抑制，翻译装置的轻微改变很容易被检测到。我们的研究结果将结合过去和近期的报告进行讨论。
