牛蛙中的尿苷二磷酸葡萄糖醛酸基转移酶活性与胆红素结合作用
UDP-glucuronosyltransferase activity and bilirubin conjugation in the bullfrog.
作者信息
Cole K D, Little G H
出版信息
Biochem J. 1983 May 15;212(2):265-9. doi: 10.1042/bj2120265.
Abstract
Bile pigments of bile and serum of Rana catesbeiana were investigated by means of high-pressure liquid chromatography. The major pigment in both bile and serum was bilirubin IX alpha. Bilirubin UDP-glucuronosyltransferase activity was found in the livers of all animals examined, but no conjugated bilirubin was detectable in the bile. Frog bile was found to contain large amounts of beta-glucuronidase. When the beta-glucuronidase inhibitor saccharo-1,4-lactone was introduced into the gall bladder followed by an exogenous bilirubin load, bilirubin glucuronide appeared in the bile.
摘要
采用高压液相色谱法对牛蛙的胆汁和血清中的胆色素进行了研究。胆汁和血清中的主要色素均为胆红素IXα。在所检查的所有动物肝脏中均发现了胆红素UDP - 葡萄糖醛酸基转移酶活性，但在胆汁中未检测到结合胆红素。发现蛙胆汁中含有大量的β - 葡萄糖醛酸酶。当将β - 葡萄糖醛酸酶抑制剂1,4 - 内酯糖引入胆囊，随后给予外源性胆红素负荷时，胆汁中出现了胆红素葡萄糖醛酸酯。
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