视黄酸对体外培养的粒细胞中磷脂酰化作用的影响。

Effect of retinoic acid on the acylation of phospholipids in granulocytes in vitro.

作者信息

Tou J

出版信息

Biochem Biophys Res Commun. 1983 Jul 18;114(1):261-7. doi: 10.1016/0006-291x(83)91622-4.

Abstract

The influence of retinoic acid on the incorporation of [1-14C]palmitic acid and [1-14C]arachidonic acid into phospholipids was examined in guinea pig peritoneal granulocytes. All-trans-retinoic acid inhibited the incorporation of both fatty acids into phosphatidic acid and phosphatidylinositol. However, it stimulated the incorporation of both fatty acids into phosphatidylcholine but not other phospholipids. All-trans-retinoic acid was more effective than 13-cis-retinoic acid. The influence of all-trans-retinoic acid on the acylation of phospholipids was concentration-dependent with significant effect occurring at 2.1 microM. The loss of labeled fatty acids from prelabeled phospholipids and the transport of labeled fatty acids into granulocytes were not responsive to the presence of retinoic acid in the incubation media. These results suggest that retinoic acid may affect the activities of acyltransferases involved in the synthesis of phosphatidic acid, phosphatidylinositol and phosphatidylcholine.

摘要

在豚鼠腹膜粒细胞中研究了视黄酸对[1-¹⁴C]棕榈酸和[1-¹⁴C]花生四烯酸掺入磷脂的影响。全反式视黄酸抑制这两种脂肪酸掺入磷脂酸和磷脂酰肌醇。然而，它刺激这两种脂肪酸掺入磷脂酰胆碱，但不影响其他磷脂。全反式视黄酸比13-顺式视黄酸更有效。全反式视黄酸对磷脂酰化的影响呈浓度依赖性，在2.1微摩尔时出现显著作用。预标记磷脂中标记脂肪酸的损失以及标记脂肪酸向粒细胞的转运对孵育培养基中视黄酸的存在无反应。这些结果表明视黄酸可能影响参与磷脂酸、磷脂酰肌醇和磷脂酰胆碱合成的酰基转移酶的活性。