谷胱甘肽S-转移酶与人类红细胞膜的钙依赖性结合。
Calcium-dependent association of glutathione S-transferase with the human erythrocyte membrane.
作者信息
White P H, Plishker G A
出版信息
Biochem Biophys Res Commun. 1983 Jul 29;114(2):488-92. doi: 10.1016/0006-291x(83)90806-9.
PMID:6411089
Abstract
Elevations in intracellular calcium increase the adsorption of a cytoplasmic protein to human red blood cell membrane. This protein migrates on SDS polyacrylamide gels at 23,000 daltons and has been called band 8. The association of this protein with the membrane is increased in sickle cell anemia. This protein is extracted from the membrane with EGTA, a calcium chelator. Enzymatic and immunological studies identify band 8 as a glutathione S-transferase.
摘要
细胞内钙水平升高会增加一种细胞质蛋白与人红细胞膜的吸附。这种蛋白在SDS聚丙烯酰胺凝胶上以23,000道尔顿的分子量迁移，被称为8带。在镰状细胞贫血中，这种蛋白与膜的结合增加。这种蛋白可被钙螯合剂EGTA从膜上提取出来。酶学和免疫学研究确定8带为谷胱甘肽S-转移酶。
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