采用高效液相色谱法、荧光光谱法和放射免疫分析法对人体体液中的麦角酸二乙酰胺（LSD）进行分析。

Analysis of LSD in human body fluids by high-performance liquid chromatography, fluorescence spectroscopy and radioimmunoassay.

作者信息

Twitchett P J, Fletcher S M, Sullivan A T, Moffat A C

出版信息

J Chromatogr. 1978 Mar 11;150(1):73-84. doi: 10.1016/s0021-9673(01)92097-7.

DOI:10.1016/s0021-9673(01)92097-7

Abstract

A scheme of analysis is described in which the particular advantages of high-performance liquid chromatography (HPLC), fluorescence spectroscopy and radioimmunoassay (RIA) are exploited to the greatest effect. RIA affords a rapid and sensitive preliminary screening method, while the subsequent HPLC analysis using fluorimetric detection yields quantitative chromatographic evidence together with characteristic fluorescence spectra. Fractionation of samples by HPLC followed by RIA of the fractions gives further confirmation of the presence of LSD and its metabolites. The combined methodology has been applied to the analysis of LSD in body fluids for forensic and clinical purposes. Levels down to 0.5 ng of LSD per ml can be detected using the minimum of sample.

摘要

本文描述了一种分析方案，该方案充分利用了高效液相色谱（HPLC）、荧光光谱法和放射免疫分析（RIA）的独特优势。放射免疫分析提供了一种快速且灵敏的初步筛选方法，而随后使用荧光检测的高效液相色谱分析则能产生定量的色谱证据以及特征荧光光谱。通过高效液相色谱对样品进行分离，然后对各馏分进行放射免疫分析，进一步证实了麦角酸二乙胺（LSD）及其代谢产物的存在。这种联合方法已应用于法医和临床目的的体液中LSD分析。使用最少的样品即可检测到低至每毫升0.5纳克的LSD水平。