Francom P, Andrenyak D, Lim H K, Bridges R R, Foltz R L, Jones R T
Center for Human Toxicology, University of Utah, Salt Lake City 84112.
J Anal Toxicol. 1988 Jan-Feb;12(1):1-8. doi: 10.1093/jat/12.1.1.
A procedure for the determination of LSD (lysergic acid diethylamide) in urine at concentrations as low as 0.5 ng/ml is presented. After addition of deuterium-labeled LSD as the internal standard, a rapid n-butyl chloride extraction of LSD from urine at pH 8 is followed by formation of the trimethylsilyl (TMS) derivative by treatment with N,O-bis(trimethylsilyl)trifluoroacetamide. The TMS derivative of LSD is identified and quantified by selected ion monitoring with a fused-silica capillary column and electron impact ionization. The procedure was used to monitor LSD concentrations in urine for eight hours following oral administration of 70.5 micrograms of LSD to two human volunteers. Concentrations of LSD determined by the assay are compared with concentrations determined by two other methods of analysis, a radioimmunoassay and a high-performance liquid chromatographic (HPLC) assay. Data concerning the stability of LSD in urine are also presented.
本文介绍了一种用于测定尿液中浓度低至0.5 ng/ml的麦角酸二乙酰胺（LSD）的方法。加入氘标记的LSD作为内标后，在pH 8的条件下用正丁基氯从尿液中快速萃取LSD，然后用N,O-双（三甲基硅基）三氟乙酰胺处理形成三甲基硅基（TMS）衍生物。通过使用熔融石英毛细管柱和电子轰击电离进行选择离子监测，对LSD的TMS衍生物进行鉴定和定量。该方法用于监测两名人类志愿者口服70.5微克LSD后八小时内尿液中的LSD浓度。将该测定法测定的LSD浓度与另外两种分析方法（放射免疫测定法和高效液相色谱（HPLC）测定法）测定的浓度进行比较。还提供了有关LSD在尿液中稳定性的数据。
