Norrod E P, Burnham J S, Williams R P, Ding M J
Can J Microbiol. 1983 May;29(5):584-92. doi: 10.1139/m83-091.
Growth of Neisseria gonorrhoeae strain F62 on medium containing pyruvate and a high ratio of cysteine to cystine resulted in functional and structural changes that are consistent with phenotypic changes in lipopolysaccharide. Both transparent (O-) and moderately opaque (O+) variants became more sensitive to killing by normal human serum and resistant to killing by pyocin G, a bacteriocin from Pseudomonas aeruginosa. Electrophoresis of outer membranes in the presence of sodium dodecyl sulfate demonstrated differences also dependent upon the growth medium. When gels were treated with periodic acid and stained with silver, lanes containing outer membranes obtained after growth in the modified medium demonstrated two bands in addition to those independent of the growth medium. The enhancement of these additional bands by periodate treatment indicated that they represent material containing carbohydrate. The mechanism by which the changes in the growth medium affected the surface of N. gonorrhoeae is not known; however, the changes demonstrated by electrophoresis were dependent upon either the high concentration of cysteine or the high ratio of cysteine to cystine.
淋病奈瑟菌F62菌株在含有丙酮酸以及高半胱氨酸与胱氨酸比例的培养基上生长，导致了功能和结构的变化，这些变化与脂多糖的表型变化一致。透明（O-）和中度不透明（O+）变体对正常人血清杀伤变得更加敏感，而对来自铜绿假单胞菌的细菌素绿脓菌素G的杀伤具有抗性。在十二烷基硫酸钠存在下对外膜进行电泳也显示出差异，这种差异也取决于生长培养基。当凝胶用高碘酸处理并用银染色时，在改良培养基中生长后获得的含有外膜的泳道除了那些与生长培养基无关的条带外，还显示出两条条带。高碘酸盐处理增强了这些额外的条带，表明它们代表含碳水化合物的物质。生长培养基的变化影响淋病奈瑟菌表面的机制尚不清楚；然而，电泳显示的变化取决于高浓度的半胱氨酸或半胱氨酸与胱氨酸的高比例。
