Norrod E P
Can J Microbiol. 1984 Oct;30(10):1297-301. doi: 10.1139/m84-207.
Addition of pyruvate to growth medium failed to induce the changes in Neisseria gonorrhoeae that have been reported previously. Addition of 3.8 mM sulfite or 1.3 mM sulfite plus 14 mM pyruvate restored the medium's reactivity in a test for cysteine and its ability to induce changes in N. gonorrhoeae. The induced changes that were restored were (i) increased colonial opacity and roughness, (ii) increased sensitivity to killing by normal human serum, and (iii) electrophoretic changes that may represent changes in lipopolysaccharide. Further characterization of the electrophoretic changes showed that the bands were resistant to treatment with proteinase K, that they were not affected by EDTA and urea, and that they were not dependent upon the stage of growth.
向生长培养基中添加丙酮酸未能诱导先前报道的淋病奈瑟菌发生变化。添加3.8 mM亚硫酸盐或1.3 mM亚硫酸盐加14 mM丙酮酸可恢复培养基在半胱氨酸测试中的反应性及其诱导淋病奈瑟菌发生变化的能力。恢复的诱导变化包括：（i）菌落透明度和粗糙度增加；（ii）对正常人血清杀伤的敏感性增加；（iii）电泳变化，可能代表脂多糖的变化。对电泳变化的进一步表征表明，这些条带对蛋白酶K处理具有抗性，不受EDTA和尿素影响，且不依赖于生长阶段。
