Janssen P T, van Bijsterveld O P
Graefes Arch Clin Exp Ophthalmol. 1983;220(4):171-4. doi: 10.1007/BF02186663.
A radial immunodiffusion assay for tear lactoferrin is described. Tear samples, collected on filter paper discs, could be applied directly to immunodiffusion plates after blotting. In 58 tear samples from healthy subjects an average lactoferrin concentration of 1.42 g/l was found. A high degree of correlation was found between the lactoferrin radial immunodiffusion and the lysozyme agar diffusion assay in a heterogeneous group, ranging from healthy subjects to patients with severe keratoconjunctivitis sicca. A lower limit of normal (0.78 g/l) could be determined for the tear lactoferrin concentration. The assay does not require laboratory facilities and therefore is an easily accessible alternative for or an addition to lacrimal gland function tests currently used.
本文描述了一种用于检测泪液乳铁蛋白的放射免疫扩散测定法。收集在滤纸圆盘上的泪液样本，在吸干后可直接应用于免疫扩散板。在58例健康受试者的泪液样本中，乳铁蛋白的平均浓度为1.42g/l。在从健康受试者到重度干燥性角结膜炎患者的异质组中，发现乳铁蛋白放射免疫扩散与溶菌酶琼脂扩散测定之间存在高度相关性。可以确定泪液乳铁蛋白浓度的正常下限为（0.78g/l）。该测定法不需要实验室设备，因此是目前使用的泪腺功能测试的一种易于获取的替代方法或补充方法。
