Milla P J, Kilby A, Rassam U B, Ersser R, Harries J T
Gut. 1983 Sep;24(9):818-24. doi: 10.1136/gut.24.9.818.
In this study a perfusion technique has been used to investigate in vivo jejunal absorption and transmural potential difference evoked by the neutral amino acids phenylalanine (56 or 20 mmol/l) and glycine (20 mmol/l), the dibasic amino acid lysine (56 or 5 mmol/l), and a dipeptide glycyl-l-phenylalanine (20 mmol/l) in 11 children with pancreatic insufficiency due to cystic fibrosis and in three children with other causes of exocrine pancreatic insufficiency. Net absorption and potential difference evoked by phenylalanine in both cystic fibrosis and pancreatic insufficiency, and net absorption of glycine in cystic fibrosis were significantly reduced; but the absorption of lysine and glycyl-l-phenylalanine was normal. Absorption of the constituent amino acids from the dipeptide was normal or increased in cystic fibrosis. Thus, these studies show a defect in active absorption of neutral amino acids in cystic fibrosis with pancreatic insufficiency and exocrine pancreatic insufficiency. We speculate that pancreatic factors participate in neutral amino acid absorption.
在本研究中，采用灌注技术，对11例因囊性纤维化导致胰腺功能不全的儿童以及3例因其他原因导致外分泌性胰腺功能不全的儿童，研究了中性氨基酸苯丙氨酸（56或20 mmol/L）、甘氨酸（20 mmol/L）、二元氨基酸赖氨酸（56或5 mmol/L）以及二肽甘氨酰-L-苯丙氨酸（20 mmol/L）在体内引起的空肠吸收和跨壁电位差。苯丙氨酸在囊性纤维化和胰腺功能不全患者中引起的净吸收和电位差，以及囊性纤维化患者中甘氨酸的净吸收均显著降低；但赖氨酸和甘氨酰-L-苯丙氨酸的吸收正常。在囊性纤维化患者中，二肽中组成氨基酸的吸收正常或增加。因此，这些研究表明，在伴有胰腺功能不全和外分泌性胰腺功能不全的囊性纤维化患者中，中性氨基酸的主动吸收存在缺陷。我们推测胰腺因素参与中性氨基酸的吸收。
