• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过单克隆抗体和免疫电子显微镜揭示的被膜小窝、细胞连接以及整个细胞表面的糖蛋白。

Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy.

作者信息

Murphy T L, Decker G, August J T

出版信息

J Cell Biol. 1983 Aug;97(2):533-41. doi: 10.1083/jcb.97.2.533.

DOI:10.1083/jcb.97.2.533
PMID:6411735
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2112517/
Abstract

Topographical descriptions of three major plasma membrane glycoproteins of murine 3T3 cells were obtained by immunoelectron microscopy with monoclonal antibodies. A glycoprotein of Mr 80,000 was distributed throughout the total cell surface. A second of Mr 90,000 was concentrated in coated pits, and a third of Mr 100,000 was localized at cell junctions.

摘要

通过使用单克隆抗体的免疫电子显微镜技术，获得了小鼠3T3细胞三种主要质膜糖蛋白的拓扑学描述。一种分子量为80,000的糖蛋白分布在整个细胞表面。第二种分子量为90,000的糖蛋白集中在被膜小窝中，第三种分子量为100,000的糖蛋白定位在细胞连接处。

相似文献

1
Glycoproteins of coated pits, cell junctions, and the entire cell surface revealed by monoclonal antibodies and immunoelectron microscopy.通过单克隆抗体和免疫电子显微镜揭示的被膜小窝、细胞连接以及整个细胞表面的糖蛋白。
J Cell Biol. 1983 Aug;97(2):533-41. doi: 10.1083/jcb.97.2.533.
2
Studies on the development and maintenance of epithelial cell surface polarity with monoclonal antibodies.利用单克隆抗体对上皮细胞表面极性的发育与维持进行的研究。
J Cell Biol. 1984 May;98(5):1777-87. doi: 10.1083/jcb.98.5.1777.
3
Membranous structures transfer cell surface proteins across NK cell immune synapses.膜性结构通过自然杀伤细胞免疫突触转运细胞表面蛋白。
Traffic. 2007 Sep;8(9):1190-204. doi: 10.1111/j.1600-0854.2007.00603.x. Epub 2007 Jul 1.
4
Monoclonal antibodies against a glycoprotein localized in coated pits and endocytic vesicles inhibit alpha 2-macroglobulin binding and uptake.针对定位于被膜小窝和内吞小泡中的一种糖蛋白的单克隆抗体，可抑制α2-巨球蛋白的结合与摄取。
J Biol Chem. 1986 Dec 15;261(35):16732-7.
5
Lateral diffusion of an 80,000-dalton glycoprotein in the plasma membrane of murine fibroblasts: relationships to cell structure and function.一种80,000道尔顿糖蛋白在小鼠成纤维细胞质膜中的侧向扩散：与细胞结构和功能的关系。
J Cell Biol. 1984 Nov;99(5):1624-33. doi: 10.1083/jcb.99.5.1624.
6
Induction of caveolae in the apical plasma membrane of Madin-Darby canine kidney cells.诱导犬肾细胞（Madin-Darby canine kidney cells）顶端质膜中小窝的形成。
J Cell Biol. 2000 Feb 21;148(4):727-39. doi: 10.1083/jcb.148.4.727.
7
Exocytotic exposure and recycling of membrane antigens of chromaffin granules: ultrastructural evaluation after immunolabeling.嗜铬粒细胞膜抗原的胞吐暴露与再循环：免疫标记后的超微结构评估
J Cell Biol. 1986 Feb;102(2):510-5. doi: 10.1083/jcb.102.2.510.
8
Topographical distribution of coated pits.被膜小窝的拓扑分布。
Nature. 1980 Aug 14;286(5774):727-9. doi: 10.1038/286727a0.
9
Redistribution of a major cell surface glycoprotein during cell movement.一种主要细胞表面糖蛋白在细胞移动过程中的重新分布。
J Cell Biol. 1984 Nov;99(5):1613-23. doi: 10.1083/jcb.99.5.1613.
10
Cell surface glycoproteins of CHO cells. II. Surface distribution and pathway of internalization.中国仓鼠卵巢细胞的细胞表面糖蛋白。II. 表面分布及内化途径。
Exp Cell Res. 1986 Jul;165(1):92-106. doi: 10.1016/0014-4827(86)90535-5.

引用本文的文献

1
Ultrasound in the screening of patients with cirrhosis with large varices.超声在肝硬化伴大静脉曲张患者筛查中的应用
Br Med J (Clin Res Ed). 1983 Aug 20;287(6391):533. doi: 10.1136/bmj.287.6391.533.
2
Lateral diffusion of an 80,000-dalton glycoprotein in the plasma membrane of murine fibroblasts: relationships to cell structure and function.一种80,000道尔顿糖蛋白在小鼠成纤维细胞质膜中的侧向扩散：与细胞结构和功能的关系。
J Cell Biol. 1984 Nov;99(5):1624-33. doi: 10.1083/jcb.99.5.1624.
3
Redistribution of a major cell surface glycoprotein during cell movement.一种主要细胞表面糖蛋白在细胞移动过程中的重新分布。
J Cell Biol. 1984 Nov;99(5):1613-23. doi: 10.1083/jcb.99.5.1613.
4
Characterization of plasma-membrane glycoproteins from functional domains of the rat hepatocyte.大鼠肝细胞功能域的质膜糖蛋白特性分析
Biochem J. 1985 Apr 15;227(2):565-72. doi: 10.1042/bj2270565.
5
The role of membrane-membrane interactions in the regulation of endothelial cell growth.膜-膜相互作用在内皮细胞生长调节中的作用。
J Cell Biol. 1985 Jun;100(6):1934-40. doi: 10.1083/jcb.100.6.1934.
6
Analysis of lateral redistribution of a plasma membrane glycoprotein-monoclonal antibody complex [corrected].质膜糖蛋白-单克隆抗体复合物侧向再分布的分析[已校正]
J Cell Biol. 1988 Feb;106(2):329-43. doi: 10.1083/jcb.106.2.329.
7
Localization of a putative cell adhesion molecule (gp110) in Wistar and Fischer rat tissues.一种假定的细胞粘附分子（gp110）在Wistar大鼠和Fischer大鼠组织中的定位。
Histochemistry. 1989;93(1):55-61. doi: 10.1007/BF00266847.
8
Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures.针对Pgp-1/CD44的单克隆抗体可阻断长期骨髓培养中的淋巴细胞生成和造血过程。
J Exp Med. 1990 Feb 1;171(2):477-88. doi: 10.1084/jem.171.2.477.
9
Dynamics and longevity of the glycolipid-anchored membrane protein, Thy-1.糖脂锚定膜蛋白Thy-1的动态变化与寿命
J Cell Biol. 1990 May;110(5):1525-31. doi: 10.1083/jcb.110.5.1525.

本文引用的文献

1
YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L.埃及伊蚊卵母细胞中的卵黄蛋白摄取
J Cell Biol. 1964 Feb;20(2):313-32. doi: 10.1083/jcb.20.2.313.
2
Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.细胞化学与电子显微镜术。醛类固定对细胞超微结构及酶活性的保存作用。
J Cell Biol. 1963 Apr;17(1):19-58. doi: 10.1083/jcb.17.1.19.
3
Characterization of plasma membrane proteins identified by monoclonal antibodies.通过单克隆抗体鉴定的质膜蛋白的特性分析
J Biol Chem. 1981 Jan 25;256(2):664-71.
4
Purification and reconstitution of HeLa cell microtubules.HeLa细胞微管的纯化与重组。
Biochemistry. 1980 Aug 19;19(17):4116-23. doi: 10.1021/bi00558a033.
5
Cell-cell adhesion molecule: identification of a glycoprotein relevant to the Ca2+-independent aggregation of Chinese hamster fibroblasts.细胞间黏附分子：一种与中国仓鼠成纤维细胞非钙依赖性聚集相关的糖蛋白的鉴定。
Cell. 1980 Jun;20(2):363-71. doi: 10.1016/0092-8674(80)90622-4.
6
Amino acid sequence of the carboxyl-terminal hydrophilic region of the H-2Kb MHC alloantigen. Completion of the entire primary structure of the H-2Kb molecule.H-2Kb主要组织相容性复合体同种异体抗原羧基末端亲水区域的氨基酸序列。H-2Kb分子完整一级结构的完成。
Biochemistry. 1981 Oct 13;20(21):5940-5. doi: 10.1021/bi00524a003.
7
Intercellular adhesion of rat hepatocytes. Identification of a cell surface glycoprotein involved in the initial adhesion process.大鼠肝细胞的细胞间黏附。参与初始黏附过程的一种细胞表面糖蛋白的鉴定。
J Biol Chem. 1982 Jun 25;257(12):6788-95.
8
Murine cell surface glycoproteins. Identification, purification, and characterization of a major glycosylated component of 110,000 daltons by use of a monoclonal antibody.小鼠细胞表面糖蛋白。利用单克隆抗体对一种110,000道尔顿主要糖基化成分进行鉴定、纯化及特性分析。
J Biol Chem. 1982 Apr 10;257(7):3970-7.
9
Coated pits act as molecular filters.有被小窝起着分子过滤器的作用。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4156-9. doi: 10.1073/pnas.77.7.4156.
10
Role of coated vesicles, microfilaments, and calmodulin in receptor-mediated endocytosis by cultured B lymphoblastoid cells.包被小泡、微丝和钙调蛋白在培养的B淋巴母细胞受体介导的内吞作用中的作用。
J Cell Biol. 1980 Oct;87(1):132-41. doi: 10.1083/jcb.87.1.132.