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[用15N标记小麦对结肠造口产蛋母鸡代谢的研究。5. 15N在肠道内容物、胃组织和肠段中的掺入与分布]

[Investigations of the metabolism of colostomised laying hens with 15N-labelled wheat. 5. Incorporation and distribution of 15N in the contents of the intestinal tract, the tissue of stomachs and the segments of the intestines].

作者信息

Gruhn K

出版信息

Arch Tierernahr. 1980 Jun;30(6):511-8. doi: 10.1080/17450398009425638.

Abstract

In an experiement with 3 colostomised laying hybrids each animal received 80 g pelleted mixed feed and 40 g 15N-labelled wheat with 20.13 atom-% 15N-excess (15N') over a period of four days. On the following four days the hens received rations composed in the same way with uulabelled wheat, however. Of the individual tissues and organs extracted from the slaughtered hens 15N' was determined in the total N and the amino acids lysine, histidine and arginine in the individual segments both of the gasto-intestinal tract and in its contents. On a average, the amount of 15N' in the stomachs, the small intestines and the colon was 43.7%, 27.2% and 29.1%. The tissue of the small intestines contained, on an average, the highest 15N' in lysine of all the basic amino acids. It amounts to 0.82 atom-% 15N' for lysine, O.55% for histidine and 0.63 atom-% 15N' for arginine. On an average, the percentage of the 15N' of the basic amino acids from the corresponding total 15N'-amount of the charges is in the contents of the gasto-intestinal tract 20.5%, in the stomach tissue 28.0% and in the tissue of the small intestines 24.4%, of the caecum 21.5% and of the rectum 25.7%.

摘要

在一项对3只结肠造口杂交蛋鸡的实验中,每只动物在4天时间内接受了80克颗粒状混合饲料和40克含20.13原子百分比过量15N(15N')的15N标记小麦。然而,在接下来的4天里,母鸡接受的是用未标记小麦以相同方式配制的日粮。从屠宰的母鸡身上提取各个组织和器官,测定其中总氮以及胃肠道及其内容物各段中赖氨酸、组氨酸和精氨酸中的15N'。平均而言,胃、小肠和结肠中的15N'含量分别为43.7%、27.2%和29.1%。小肠组织中,所有碱性氨基酸中赖氨酸的平均15N'含量最高。赖氨酸的15N'含量为0.82原子百分比,组氨酸为0.55%,精氨酸为0.63原子百分比。平均而言,胃肠道内容物中碱性氨基酸的15N'占相应总15N'含量的百分比为20.5%,胃组织中为28.0%,小肠组织中为24.4%,盲肠中为21.5%,直肠中为25.7%。

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