由胶原蛋白赖氨酸羟化酶催化的反应中氧气的来源。
The source of oxygen in the reaction catalysed by collagen lysyl hydroxylase.
作者信息
Kikuchi Y, Suzuki Y, Tamiya N
出版信息
Biochem J. 1983 Aug 1;213(2):507-12. doi: 10.1042/bj2130507.
The synthetic peptides (Pro-Pro-Gly)5 and (Ile-Lys-Gly)5-Phe were hydroxylated with collagen prolyl hydroxylase and lysyl hydroxylase in an 18O2 atmosphere. The oxygen atoms in the hydroxy groups of hydroxyproline and hydroxylysine were 87% and 6.5% respectively derived from the atmospheric 18O2. The results are consistent with those reported previously for proline hydroxylation in vivo [Fujimoto & Tamiya (1962) Biochem. J. 84, 333-335; Prockop, Kaplan & Udenfriend (1962) Biochem. Biophys. Res. Commun. 9, 192-196; Fujimoto & Tamiya (1963) Biochem. Biophys. Res. Commun. 10, 498-501; Prockop, Kaplan & Udenfriend (1963) Arch. Biochem. Biophys. 101, 499-503] and in vitro [Cardinale, Rhoads & Udenfriend (1971) Biochem. Biophys. Res. Commun. 43, 537-543] and for lysine hydroxylation in vivo [Fujimoto & Tamiya (1963) Biochem. Biophys. Res. Commun. 10, 498-501]. In view of the similarities of these two oxygenase-type hydroxylation reactions the participation of intermediates is proposed, the oxygen atoms of which are exchangeable with those of water. The atmospheric oxygen atoms incorporated into the intermediate must be equilibrated with water oxygen atoms in the slower lysyl hydroxylase reaction.
合成肽（脯氨酸 - 脯氨酸 - 甘氨酸）5和（异亮氨酸 - 赖氨酸 - 甘氨酸）5 - 苯丙氨酸在18O2气氛中被胶原蛋白脯氨酰羟化酶和赖氨酰羟化酶羟基化。羟脯氨酸和羟赖氨酸羟基中的氧原子分别有87%和6.5%来自大气中的18O2。这些结果与先前报道的体内脯氨酸羟基化[藤本和民谷（1962年）《生物化学杂志》84卷，333 - 335页；普罗科普、卡普兰和乌登弗里德（1962年）《生物化学与生物物理研究通讯》9卷，192 - 196页；藤本和民谷（1963年）《生物化学与生物物理研究通讯》10卷，498 - 501页；普罗科普、卡普兰和乌登弗里德（1963年）《生物化学与生物物理学报》101卷，499 - 503页]以及体外[卡尔迪纳莱、罗兹和乌登弗里德（1971年）《生物化学与生物物理研究通讯》43卷，537 - 543页]和体内赖氨酸羟基化[藤本和民谷（1963年）《生物化学与生物物理研究通讯》10卷，498 - 501页]的结果一致。鉴于这两种加氧酶型羟基化反应的相似性，提出了中间体的参与，其中间体的氧原子可与水的氧原子交换。在较慢的赖氨酰羟化酶反应中，掺入中间体的大气氧原子必须与水的氧原子达到平衡。
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