Böhlen P, Brazeau P, Bloch B, Ling N, Gaillard R, Guillemin R
Biochem Biophys Res Commun. 1983 Aug 12;114(3):930-6. doi: 10.1016/0006-291x(83)90649-6.
Human hypothalamic growth hormone-releasing factor (GRF) was purified by gel filtration and reverse-phase HPLC. Bioassay and two radioimmunoassays of different specificity revealed the presence of two major forms of GRF-activity which coelute with human pancreas GRFs, hpGRF-44-NH2 and hpGRF-40 previously characterized in pancreas tumors. The bioactive material coeluting with hpGRF-44-NH2 is recognized by two antibodies which are directed against the amidated COOH-terminal sequence and the central portion of the GRF-44 peptide. The bioactive GRF which coelutes with hpGRF-40 reacts only with the antibody directed against the central portion of hpGRF. These data strongly suggest that the human hypothalamus contains the same major forms of GRF that were identified in pancreas tumors responsible for acromegaly in the absence of a pituitary tumor.
人下丘脑生长激素释放因子（GRF）通过凝胶过滤和反相高效液相色谱法进行纯化。生物测定以及两种具有不同特异性的放射免疫测定表明，存在两种主要形式的GRF活性，它们与人类胰腺GRF（hpGRF）共洗脱，即先前在胰腺肿瘤中鉴定出的hpGRF-44-NH2和hpGRF-40。与hpGRF-44-NH2共洗脱的生物活性物质可被两种抗体识别，这两种抗体分别针对酰胺化的COOH末端序列和GRF-44肽的中央部分。与hpGRF-40共洗脱的生物活性GRF仅与针对hpGRF中央部分的抗体发生反应。这些数据强烈表明，人类下丘脑含有与在无垂体肿瘤的肢端肥大症胰腺肿瘤中鉴定出的相同主要形式的GRF。
