Ling N, Esch F, Böhlen P, Brazeau P, Wehrenberg W B, Guillemin R
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4302-6. doi: 10.1073/pnas.81.14.4302.
The hypophysiotropic peptide, growth hormone-releasing factor (GRF), was isolated from human hypothalamic-hypophysial tissues by means of acid extraction, immunoaffinity chromatography, gel filtration, and two steps of reverse-phase high-performance liquid chromatography. Amino acid sequence determination using a gas-phase sequencer and reverse-phase liquid chromatography of the native peptide and its synthetic replicates showed its primary structure to be as follows: H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln -Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser -Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu-NH2, which is identical to that of the GRF recently isolated and characterized from a human pancreatic tumor that had caused acromegaly. Human hypothalamic GRF shows major homologies (93%, 89%, and 86%, respectively) when its primary structure is compared to that of the hypothalamic GRF from the porcine, bovine, caprine, and ovine species.
促垂体肽,生长激素释放因子(GRF),是通过酸提取、免疫亲和色谱、凝胶过滤以及两步反相高效液相色谱从人下丘脑-垂体组织中分离出来的。使用气相测序仪以及对天然肽及其合成复制品进行反相液相色谱分析来确定氨基酸序列,结果显示其一级结构如下:H-Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln -Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser -Asn-Gln-Glu-Arg-Gly-Ala-Arg-Ala-Arg-Leu-NH2,这与最近从导致肢端肥大症的人胰腺肿瘤中分离并鉴定出的GRF的结构相同。当将人下丘脑GRF的一级结构与来自猪、牛、山羊和绵羊物种的下丘脑GRF的一级结构进行比较时,人下丘脑GRF分别显示出主要的同源性(分别为93%、89%和86%)。