Sakai K, Salvert D, Kitahama K, Kimura H, Maeda T, Jouvet M
C R Seances Acad Sci III. 1983;296(21):1013-8.
Through combined retrograde labeling with HRP and serotonin immunohistochemistry, we have demonstrated that ventral and caudal hypothalamic neurons, both non-immunoreactive and immunoreactive to serotonin after administration of 5-hydroxytryptophane, give rise to direct ascending projections to the entire cerebral cortex as well as direct descending projections to the nucleus locus coeruleus alpha in the pontine tegmentum. A retrograde double labeling method with fluorescent dyes further suggested that the population of ascending hypothalamic neurons is different from that of descending ones.
通过将HRP逆行标记与5-羟色胺免疫组织化学相结合，我们已经证明，下丘脑腹侧和尾侧神经元，无论是在给予5-羟色氨酸后对5-羟色胺无免疫反应还是有免疫反应的，都产生直接向上投射至整个大脑皮层以及直接向下投射至脑桥被盖中的蓝斑α核。一种使用荧光染料的逆行双重标记方法进一步表明，下丘脑向上投射神经元的群体与向下投射神经元的群体不同。
