Mano Y, Sawasaki Y, Takahashi K, Goto T
Experientia. 1983 Oct 15;39(10):1144-6. doi: 10.1007/BF01943152.
We have developed a simple method for the isolation of endothelial cells from human umbilical artery. The method provides a sufficient number of cells to be of experimental value. The presence of factor VIII antigen specific for endothelium has been demonstrated by immunofluorescence as well as by the peroxidase-antiperoxidase immune complex method.
我们已经开发出一种从人脐动脉中分离内皮细胞的简单方法。该方法能提供足够数量具有实验价值的细胞。通过免疫荧光以及过氧化物酶 - 抗过氧化物酶免疫复合物方法已经证实了内皮特异性因子VIII抗原的存在。