Chow V, Murray R K, Dixon J D, Kurosky A
FEBS Lett. 1983 Mar 21;153(2):275-9. doi: 10.1016/0014-5793(83)80623-1.
The primary translation product of the mRNA for rabbit haptoglobin was obtained from a rabbit reticulocyte lysate cell-free system by immunoprecipitation with an antiserum that was directed to the beta chain of haptoglobin. Analysis of the translation product by gel electrophoresis and by protein sequencing analysis identified a single polypeptide of Mr 41 000. Sequence analysis established a signal region of 18 residues that was immediately followed by the alpha chain sequence. These results give strong evidence that haptoglobin is initially synthesized as a single chain composed of a signal peptide followed by alpha and beta chain regions, respectively.
兔触珠蛋白mRNA的主要翻译产物是通过用针对触珠蛋白β链的抗血清进行免疫沉淀，从兔网织红细胞裂解物无细胞系统中获得的。通过凝胶电泳和蛋白质测序分析对翻译产物进行分析，确定了一条分子量为41000的单一多肽。序列分析确定了一个由18个残基组成的信号区域，紧接着是α链序列。这些结果有力地证明，触珠蛋白最初是作为一条由信号肽、随后分别是α链和β链区域组成的单链合成的。
