Mayers G L, Bankert R B, Pressman D
J Immunol. 1978 Apr;120(4):1143-8.
The primary antibody response to dextran B1355 in BALB/c mice is largely a homogenous IgM antibody. This antibody appears to be similar if not identical to the myeloma protein, protein 104E, for the following reasons: a) isoelectric focusing of the 7S monomers, separately and together in co-isoelectric focusing, give the same pattern, both in the presence and absence of 4M urea; b) the inhibition of lysis of the dextran-coated SRBC by specifically purified anti-dextran antibody and by protein 104E required essentially the same concentration of dextran B1355. This similarity was further demonstrated by plaque assays with dextran-coated SRBC, in which the formation of plaques was inhibited by free dextran. Inhibition of plaques produced by both types of cells required essentially the same concentration of dextran B1355. On these bases, there appears to be no difference in properties (or in structure) between the myeloma protein and the induced antibody.
BALB/c小鼠对葡聚糖B1355的初次抗体反应主要是一种同质的IgM抗体。这种抗体似乎与骨髓瘤蛋白104E相似，如果不是完全相同的话，原因如下：a）7S单体的等电聚焦，无论是单独还是在共等电聚焦中一起进行，在有和没有4M尿素的情况下都给出相同的图谱；b）特异性纯化的抗葡聚糖抗体和蛋白104E对葡聚糖包被的SRBC裂解的抑制基本上需要相同浓度的葡聚糖B1355。用葡聚糖包被的SRBC进行的空斑试验进一步证明了这种相似性，其中空斑的形成被游离葡聚糖抑制。两种类型细胞产生的空斑抑制基本上需要相同浓度的葡聚糖B1355。基于这些，骨髓瘤蛋白和诱导抗体之间在性质（或结构）上似乎没有差异。
