van Mourik P, Petranyi G, Gyodi E, Lindblom B, Holmlund G, Kastelan A, van der Reijden H J, Iványi P
Tissue Antigens. 1983 Aug;22(2):134-41. doi: 10.1111/j.1399-0039.1983.tb01179.x.
A murine allo-immune A.TH anti-A.TL (anti-Iak) serum pool and sera from individual immunized mice were tested on a large panel of human DR typed B cells, obtained from peripheral blood (PBL-B cells), and human lymphoid tumour cells. The anti-Iak serum pool appeared to be a useful reagent both in cytotoxicity and immunofluorescence for the examination of the presence of class-2 HLA molecules on tumour cells. Only one discrepancy was observed when the pool was tested in parallel with a xeno-anti-human B-cell serum on the cells of 56 lymphoid tumours. Nine sera from individual mice were tested on a panel of 93 PBL-B cells. Some of these sera reacted significantly more weakly with human B cells positive for the DR5 and/or DR7 antigens.
用一组从外周血获得的人类DR分型B细胞（外周血淋巴细胞B细胞）和人类淋巴肿瘤细胞，检测了小鼠同种免疫A.TH抗A.TL（抗Iak）血清库以及来自个体免疫小鼠的血清。抗Iak血清库在细胞毒性和免疫荧光检测肿瘤细胞上的2类HLA分子方面似乎是一种有用的试剂。当该血清库与异种抗人B细胞血清在56个淋巴肿瘤细胞上平行检测时，仅观察到一处差异。用93个外周血淋巴细胞B细胞对来自个体小鼠的9份血清进行了检测。其中一些血清与DR5和/或DR7抗原阳性的人类B细胞反应明显较弱。
