一种用于释放和测定¹⁴CO₂的装置。

A device for the liberation and determination of 14CO2.

作者信息

Schadewaldt P, Förster M E, Münch U, Staib W

出版信息

Anal Biochem. 1983 Jul 15;132(2):400-4. doi: 10.1016/0003-2697(83)90026-x.

Abstract

A simple closed system for the serial determination of 14CO2 in small volumes of fluid samples, is described. The device consists of commercially available scintillation vials and silicone tube seals. 14CO2 is selectively liberated by citric acid and absorbed in a scintillation vial by Hyamine. Experiments on the effect of dichloroacetate on pyruvate dehydrogenase activity in rat hindlimbs perfused with [1-14C]pyruvate demonstrate the applicability of the method.

摘要

本文描述了一种用于小体积流体样本中14CO2连续测定的简单封闭系统。该装置由市售闪烁瓶和硅胶管密封件组成。14CO2通过柠檬酸选择性释放，并被海胺吸收在闪烁瓶中。在用[1-14C]丙酮酸灌注的大鼠后肢中，关于二氯乙酸对丙酮酸脱氢酶活性影响的实验证明了该方法的适用性。