灌注大鼠骨骼肌中丙酮酸代谢区室化的证据。
Evidence for the compartmentation of pyruvate metabolism in perfused rat skeletal muscle.
作者信息
Schadewaldt P, Münch U, Staib W
出版信息
Biochem J. 1983 Dec 15;216(3):761-4. doi: 10.1042/bj2160761.
Abstract
In rat hindlimbs perfused with [1-14C]pyruvate and 5 mM-dichloroacetate, the calculated apparent rate of pyruvate decarboxylation was decreased with increasing perfusate pyruvate concentrations. However, in the absence of dichloroacetate the apparent rate of decarboxylation increased under these conditions. Dichloroacetate enhanced [1-14C]pyruvate uptake, but decreased the specific radioactivity of effluent lactate. Glycogen metabolism remained unaffected. The results were not consistent with a common pyruvate pool, but provide evidence for the compartmentation of pyruvate metabolism.
摘要
在用[1-¹⁴C]丙酮酸和5 mM二氯乙酸灌注的大鼠后肢中，随着灌注液中丙酮酸浓度的增加，计算得出的丙酮酸脱羧表观速率降低。然而，在没有二氯乙酸的情况下，在这些条件下脱羧表观速率增加。二氯乙酸增强了[1-¹⁴C]丙酮酸的摄取，但降低了流出乳酸的比放射性。糖原代谢未受影响。结果与常见的丙酮酸池不一致，但为丙酮酸代谢的区室化提供了证据。
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