用于液体闪烁计数的过期14CO2自动收集器。

Automatic collector of expired 14CO2 for liquid scintillation counting.

作者信息

Richards A C, Shamgar F A, Collins F D

机构信息

Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.

出版信息

Anal Biochem. 1978 Nov;91(1):199-208. doi: 10.1016/0003-2697(78)90832-1.

DOI:10.1016/0003-2697(78)90832-1

PMID:9762100

Abstract

A filtration technique was employed to trap 14CO2 continuously for liquid scintillation counting. Devices for delivering scintillator and ethanolamine solutions were combined symmetrically with two fritted-glass aspirators for alternating operation. The collector was regulated by a fraction collector timer. Trial and animal tests indicated that the described method was efficient, reliable, and more convenient for frequent collection over long periods than alternative methods. The automatic collector was used for metabolic studies of [1-14C] arachidonic acid in rats kept in metabolic cages and the results were processed by multicompartmental analysis.

摘要

采用一种过滤技术连续捕获¹⁴CO₂用于液体闪烁计数。输送闪烁体和乙醇胺溶液的装置与两个烧结玻璃吸气器对称组合，以便交替运行。收集器由部分收集器定时器调节。试验和动物测试表明，所述方法高效、可靠，并且与其他方法相比，更便于长时间频繁收集。自动收集器用于在代谢笼中饲养的大鼠中对[1-¹⁴C]花生四烯酸进行代谢研究，结果通过多室分析进行处理。