Eanes W F
Biochem Genet. 1983 Aug;21(7-8):703-11. doi: 10.1007/BF00498917.
Studies were undertaken to investigate two critical aspects of the glucose-6-phosphate dehydrogenase polymorphism in Drosophila melanogaster. The first investigation unequivocally maps the genetic site of the G6PD locus to the X chromosome. The second study subjects a set of isochromosomal lines to sequential electrophoresis in an attempt to uncover common molecular heterogeneity within the global polymorphism, assuming that this variation may have gone undetected under conventional electrophoretic conditions. The genetic site was mapped following the segregation of the two common electrophoretic alleles, a so-called null allele, and two rare electrophoretic variants. From the pooled results, the Zw locus mapped to 62.9 on the X chromosome relative to the flanking markers car (at 62.5) and sw (at 64.7). A set of 126 iso-X chromosomal lines of diverse geographic origin was subjected to sequential electrophoresis under three different acrylamide conditions in addition to the conventional starch electrophoretic system. No additional variation beyond the common diallele polymorphism was seen.
开展了多项研究以调查黑腹果蝇中葡萄糖-6-磷酸脱氢酶多态性的两个关键方面。第一项研究明确地将G6PD基因座的遗传位点定位到X染色体上。第二项研究让一组等染色体系进行连续电泳，试图在整体多态性中发现常见的分子异质性，假定这种变异在传统电泳条件下可能未被检测到。通过追踪两个常见电泳等位基因、一个所谓的无效等位基因和两个罕见电泳变体的分离情况来确定遗传位点。综合结果显示，相对于侧翼标记car（位于62.5）和sw（位于64.7），Zw基因座在X染色体上的位置为62.9。除了传统的淀粉电泳系统外，在三种不同的丙烯酰胺条件下，对一组126个来自不同地理区域的异X染色体系进行了连续电泳。未发现除常见双等位基因多态性之外的其他变异。
