Mauduit G, Turbitt M, MacKie R M
Br J Dermatol. 1983 Oct;109(4):377-81. doi: 10.1111/j.1365-2133.1983.tb04611.x.
Biopsies from twenty-nine malignant skin tumours and four benign dermatoses and fourteen samples of normal skin were stained for cell surface membrane or cytoplasmic expression of HLA heavy chain and light chain (beta 2 microglobulin) by use of the immunoperoxidase technique on frozen sections using monoclonal antibodies. Results showed a loss of beta 2 microglobulin from the surface of malignant cells in twenty-four out of twenty-nine cutaneous malignancies and the presence of HLA heavy chain in all twenty-nine primary and secondary tumours. Expression of both heavy and light chains was observed in the four benign lesions and in normal epidermis in all samples examined. This would appear to be the first report of dissociation of heavy and light HLA chain expression in various forms of cutaneous malignancy.
采用免疫过氧化物酶技术，利用单克隆抗体对29例恶性皮肤肿瘤、4例良性皮肤病的活检标本以及14份正常皮肤样本的冰冻切片进行细胞表面膜或细胞质中HLA重链和轻链（β2微球蛋白）表达的染色。结果显示，29例皮肤恶性肿瘤中有24例恶性细胞表面β2微球蛋白缺失，所有29例原发性和继发性肿瘤中均存在HLA重链。在所有检测样本的4例良性病变和正常表皮中均观察到重链和轻链的表达。这似乎是关于各种皮肤恶性肿瘤中HLA重链和轻链表达解离的首次报道。
