Soltani K, Yachnin S, Brickman F
J Invest Dermatol. 1978 Apr;70(4):204-6. doi: 10.1111/1523-1747.ep12541327.
Human alpha-fetoprotein (HAFP) was demonstrated in clusters of atypical cells of an extensive Bowen's disease involving the pubic area. Frozen sections were studied by indirect immunofluorescence using monospecific rabbit anti-HAFP antiserum or purified rabbit anti-HAFP antibody followed by staining with fluorescein isothiocyanate (FITC)-labeled goat anti-rabbit gamma-globulin and by a direct technique using FITC-labeled rabbit anti-HAFP Fab2 fragments. Sections of fetal liver served as positive controls and sections of adult human skin and liver were used as negative controls. Preabsorption of the anti-HAFP antibodies with purified HAFP blocked the fluorescence. This finding adds Bowen's disease to the list of premalignant disorders which possess HAFP as an oncofetal marker of their malignant potential. HAFP might possibly enhance malignant behavior through interference with cellular immunosurveillance mechanisms.
在累及耻骨区的广泛性鲍温病的非典型细胞簇中证实了人甲胎蛋白（HAFP）。使用单特异性兔抗HAFP抗血清或纯化的兔抗HAFP抗体通过间接免疫荧光研究冰冻切片，随后用异硫氰酸荧光素（FITC）标记的山羊抗兔γ球蛋白染色，并使用FITC标记的兔抗HAFP Fab2片段通过直接技术进行研究。胎儿肝脏切片用作阳性对照，成人皮肤和肝脏切片用作阴性对照。用纯化的HAFP预吸收抗HAFP抗体可阻断荧光。这一发现使鲍温病被列入具有HAFP作为其恶性潜能的肿瘤胎儿标志物的癌前疾病名单中。HAFP可能通过干扰细胞免疫监视机制来增强恶性行为。
