van Bennekom C A, van Laarhoven J P, de Bruyn C H, Oei T L
J Clin Chem Clin Biochem. 1978 Apr;16(4):245-8. doi: 10.1515/cclm.1978.16.4.245.
A simple and sensitive radiochemical micro-assay has been developed for the determination of plasma guanase activity. The method is based upon the measurement of the conversion of 14C-labeled guanine to xanthine, catalysed by the enzyme guanase (guanine aminohydrolase;EC 3.5.4.3). Using this method, the catalytic activity in the plasma of adult healthy controls was 0.040 +/- 0.09 nmol/h.mg protein (x +/- s). In children under 5 years of age higher levels of enzyme activity were demonstrated. In adult patients with liver disease plasma guanase activities were found to be 3 to 7-fold increased as compared to the normal adult mean value.
已开发出一种简单且灵敏的放射化学微量测定法，用于测定血浆鸟嘌呤酶活性。该方法基于对由鸟嘌呤酶（鸟嘌呤氨基水解酶；EC 3.5.4.3）催化的14C标记鸟嘌呤向黄嘌呤转化的测量。使用此方法，成年健康对照者血浆中的催化活性为0.040±0.09 nmol/小时·毫克蛋白（x±s）。5岁以下儿童的酶活性水平较高。在成年肝病患者中，发现血浆鸟嘌呤酶活性比正常成年人平均值增加了3至7倍。
